| 03.08.2025 | 1. kolo | SK Fotbalová škola Třebíč 0-6 SK Artis Brno B | 90' | | | | |
| 10.08.2025 | 2. kolo | SK Artis Brno B 6-1 TJ Dálnice Speřice | 90' | | | | |
| 17.08.2025 | 3. kolo | TJ Sokol Tasovice 1-5 SK Artis Brno B | 90' | | | | |
| 24.08.2025 | 4. kolo | SK Artis Brno B 1-1 FC Havlíčkův Brod | 90' | | | | |
| 31.08.2025 | 5. kolo | FC Velké Meziříčí 1-2 SK Artis Brno B | 90' | | | | |
| 07.09.2025 | 6. kolo | FK Maraton Pelhřimov 0-2 SK Artis Brno B | 90' | | | | |
| 13.09.2025 | 7. kolo | SK Artis Brno B 0-4 FC Velká Bíteš | 90' | | | | |
| 20.09.2025 | 8. kolo | 1. SC Znojmo FK 1-0 SK Artis Brno B | 90' | | | | |
| 27.09.2025 | 9. kolo | SK Artis Brno B 4-2 FC Kuřim | 90' | | | | |
| 04.10.2025 | 10. kolo | FC Chotěboř 0-5 SK Artis Brno B | 90' | | | | |
| 11.10.2025 | 11. kolo | SK Artis Brno B 0-1 FC Sparta Brno | 90' | | | | |
| 19.10.2025 | 12. kolo | FC ŽĎAS Žďár nad Sázavou 0-4 SK Artis Brno B | 90' | | | | |
| 26.10.2025 | 13. kolo | SK Artis Brno B 1-1 SK Tatran Ždírec nad Doubravou | 90' | | | | |
| 01.11.2025 | 14. kolo | AFC Humpolec 4-5 SK Artis Brno B | 90' | | | |  |
| 09.11.2025 | 15. kolo | SK Artis Brno B 1-1 TJ Tatran Bohunice | 90' | | | | |
| 08.03.2026 | 16. kolo | SK Artis Brno B 5-1 SK Fotbalová škola Třebíč | 90' | | | | |
| 15.03.2026 | 17. kolo | TJ Dálnice Speřice 1-3 SK Artis Brno B | 90' | | | | |
| 22.03.2026 | 18. kolo | SK Artis Brno B 5-0 TJ Sokol Tasovice | 90' | | | | |
| 28.03.2026 | 19. kolo | FC Havlíčkův Brod 1-6 SK Artis Brno B | 90' | | | | |
| 05.04.2026 | 20. kolo | SK Artis Brno B 1-0 FC Velké Meziříčí | 90' | | | | |
| 12.04.2026 | 21. kolo | SK Artis Brno B 3-1 FK Maraton Pelhřimov | 90' | | | | |
| 19.04.2026 | 22. kolo | FC Velká Bíteš 0-2 SK Artis Brno B | 90' | | | | |
