| 02.08.2025 | 1. kolo | TJ Tatran Bohunice 1-1 FC Velká Bíteš | 90' | | | | |
| 08.08.2025 | 2. kolo | TJ Tatran Bohunice 1-0 1. SC Znojmo FK | 90' | | | |  |
| 23.08.2025 | 4. kolo | FC Chotěboř 0-2 TJ Tatran Bohunice | 90' | | | | |
| 30.08.2025 | 5. kolo | TJ Tatran Bohunice 2-0 FC Sparta Brno | 90' | | | | |
| 07.09.2025 | 6. kolo | FC ŽĎAS Žďár nad Sázavou 0-1 TJ Tatran Bohunice | 90' | | | | |
| 13.09.2025 | 7. kolo | TJ Tatran Bohunice 2-0 SK Tatran Ždírec nad Doubravou | 90' | | | | |
| 20.09.2025 | 8. kolo | AFC Humpolec 0-1 TJ Tatran Bohunice | 90' | | | | |
| 04.10.2025 | 10. kolo | TJ Tatran Bohunice 0-1 SK Fotbalová škola Třebíč | 21' | | | | |
| 12.10.2025 | 11. kolo | TJ Dálnice Speřice 4-0 TJ Tatran Bohunice | 90' | | | | |
| 18.10.2025 | 12. kolo | TJ Tatran Bohunice 1-1 TJ Sokol Tasovice | 90' | | | | |
| 25.10.2025 | 13. kolo | FC Havlíčkův Brod 2-1 TJ Tatran Bohunice | 56' | | | | |
| 09.11.2025 | 15. kolo | SK Artis Brno B 1-1 TJ Tatran Bohunice | 1' | | | | |
| 27.03.2026 | 19. kolo | TJ Tatran Bohunice 2-1 FC Chotěboř | 76' | | | | |
| 11.04.2026 | 21. kolo | TJ Tatran Bohunice 2-0 FC ŽĎAS Žďár nad Sázavou | 90' | | | | |
| 15.04.2026 | 18. kolo | FC Kuřim 3-1 TJ Tatran Bohunice | 90' | | | |  |
| 19.04.2026 | 22. kolo | SK Tatran Ždírec nad Doubravou 2-0 TJ Tatran Bohunice | 90' | | | | |
